|
Product Details
|
|
Product Name
|
Anti-Human IgG4 Rabbit Monoclonal Antibody [RM120]
|
|
Product Description
|
Rabbit monoclonal to Human IgG4
|
|
Catalog No.
|
31-1022-00
|
|
Clone Name
|
RM120
|
|
Product Data Sheet
|
Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
|
|
Specificity
|
RM120 reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
|
|
Immunogen
|
Peptide corresponding to the hinge region of Human IgG4
|
|
Application
|
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Western Blot
|
|
Species Reactivity
|
Human
|
|
Conjugate
|
None
|
|
Intended Use
|
Research Use Only
|
|
.
|
|
|
Properties
|
|
Form
|
Liquid
|
|
Storage Instructions
|
Store at -20.0°C
|
|
Stability
|
Stable for 1 Year at -20.0°C from date of receipt
|
|
Storage Buffer
|
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
|
|
Volume/Item Size
|
100ug
|
|
Concentration
|
1.0 mg/mL
|
|
Purity
|
Protein A affinity purified from an animal origin–free culture supernatant
|
|
Clone Type
|
Monoclonal
|
|
Isotype
|
Rabbit IgG
|
|
Usage
|
ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Western Blot (WB): 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry (ICC): 1 ug/mL-10ug/mL;
Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
|
|
Keywords
|
|
|
.
|
|
|
Product Images
|
|
Detection of human IgG4 in monkey serum.
|
|
Sandwich ELISA using RM120 as the capture
|
|
Western Blot of human, mouse, rat, and goat
|
|
A titer ELISA using RM120. The plate was coated
|
|
Immunohistochemical staining of formalin fixed
|
|
ELISA showing RM120 does not react to monkey
|
|
Immunohistochemical staining of FFPE Tonsil tissue
Image courtesy of Shaomin Hu, MD, PhD,
|
