Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant histone H3.3 (3), using RM169 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 23 in treated HeLa.
RM169 specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone RM221) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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