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Product Details
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Product Name
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Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236]
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Product Description
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Rabbit monoclonal to 5-hydroxymethylcytosine (5-hmC); 5'-hmC
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Catalog No.
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31-1111-00
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Clone Name
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RM236
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Product Data Sheet
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Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody Data Sheet RM236
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Specificity
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RM236 reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
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Immunogen
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BSA-conjugated 5-hydroxymethylcytosine.
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Application
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Hydroxymethylated DNA immunoprecipitation (hMeDIP)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
ELISA
Dot Blot (DB)
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Species Reactivity
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All
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt.
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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50 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Dot Blot: 0.2 ug/mL – 1 ug/mL;
ELISA: 0.1 ug/mL - 1 ug/mL;
IHC: 0.1 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL- 2 ug/mL;
hMeDIP: 0.2 ug/mL- 2 ug/mL.
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References for 5-hydroxymethylcytosine (5-hmC) antibody [RM236]
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J Reichmann et al. Dual spindle formation in zygotes keeps parental genomes apart in early mammalian embryos,
bioRxiv; 10.1101/198275 (2017). IF; Mouse Read more
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Product Images
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ELISA of single stranded DNA using rabbit monoclonal
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Dot blot of double stranded DNA using rabbit monoclonal
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Immunohistochemical staining of formalin fixed and
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Immunocytochemical staining of HeLa cells using
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Direct ELISA of mouse brain genomic DNA using
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hMeDIP was performed using anti-5-hmC antibody
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