RM204 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.
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