Western blot of nonreduced(-) and reduced(+) mouse IgG2aκ (20 ng/lane), using 0.2ug/mL of RevMAb clone RM107. This antibody only reacts to nonreduced Mouse IgG2aκ.
ELISA of mouse immunoglobulins shows RM107 reacts to the Fab region of mouse IgG2aκ; no cross reactivity with IgG2aκ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2aκ. The plate was coated with different amounts of mouse IgG2aκ. A serial dilution of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti- rabbit IgG as the secondary antibody.
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