Immunohistochemistry of Human Tonsil using Anti-Lambda Light Chain antibody RM127.
ELISA of human immunoglobulins shows RM127 reacts to the lambda light chain of human immuno- globulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno- globulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phos- phatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using RM127. The plate was coated with different amounts of human IgG3λ. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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