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Anti-Phosphothreonine Rabbit Monoclonal Antibody, Clone RM102

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Anti-Phosphothreonine Rabbit Monoclonal Antibody, Clone RM102

品牌：RevMAb

貨號：31-1009-00

規(guī)格：100ug

貨期：電詢

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Product Details
Product Name	Anti-Phosphothreonine Rabbit Monoclonal Antibody [RM102]
Description	Rabbit monoclonal to Phosphothreonine
Catalog No.	31-1009-00
Clone Name	RM102
Product Data Sheet	Anti-Phosphothreonine Rabbit Monoclonal Antibody Data Sheet RM102
Specificity	RM102 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Immunogen	Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide
Application	Western Blot (WB) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	All Species
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100ug
Concentration	0.5 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot (WB): 1:500 -1:2000 dilution; Immunoprecipitation (IP): 1:100-1:500 dilution; Chromatin IP (ChIP): 1:100-1:500 dilution; Immunocytochemistry (ICC): 1:100-1:500 dilution; Immunohistochemistry (IHC): 1:100-1:500 dilution.
Keywords
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Product Images
	Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using RevMAb Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).
	Western blot of serum-starved A431 cells non-treated or treated with Calyculin A/Okadaic Acid,using RevMAb Clone RM102 at 1/2000 dilution.
	Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by RevMAb clone RM102 at1/500 dilution, and then blotted with RM102.(1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.
	RevMAb rabbit monoclonal antibody clone RM102 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.