Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells.
Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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