|
Product Details
|
|
Product Name
|
Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody [RM240]
|
|
Product Description
|
Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant
|
|
Catalog No.
|
31-1120-00
|
|
Clone Name
|
RM240
|
|
Product Data Sheet
|
Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240
|
|
Specificity
|
RM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 .
|
|
Immunogen
|
A peptide corresponding to Histone H3.3 G34R mutant
|
|
Application
|
Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Chromatin IP
|
|
Species Reactivity
|
All
|
|
Conjugate
|
None
|
|
Intended Use
|
Research Use Only
|
|
.
|
|
|
Properties
|
|
Form
|
Liquid
|
|
Storage Instructions
|
Store at -20.0°C
|
|
Stability
|
Stable for 1 Year at -20.0°C from date of receipt
|
|
Storage Buffer
|
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
|
|
Volume/Size
|
50 ug
|
|
Concentration
|
1.0 mg/mL
|
|
Purity
|
Protein A affinity purified from an animal origin–free culture supernatant
|
|
Clone Type
|
Monoclonal
|
|
Isotype
|
Rabbit IgG
|
|
Usage
|
Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC): 0.5 – 5 ug/mL.
|
|
References for Histone H3.3 G34R Mutant antibody [RM240]
|
Pathania et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor
Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017)10.1016/j.ccell.2017.09.014. WB; Human. Read more (PubMed: 29107533)
Yamamoto H.et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017)10.1016/j.humpath.2017.11.020 IHC; Human. Read more (PubMed: 29241742)
|
|
.
|
|
|
Product Images
|
|
Western Blot analysis of cell lysates prepared from
|
|
Immunohistochemical staining of formalin fixed and
|
|
IHC of clinically validated FFPE Glioblastoma tumor
Image courtesy of Dr. Sebastian Brandner, UCL
|
|
IHC of clinically validated FFPE Glioblastoma tumor
Image courtesy of Dr. Sebastian Brandner, UCL
|
|
ICC staining of primary human cell lines expressing
Image courtesy of Dr. Nada Jabado, McGill University,
|
