品牌：Emfret
貨號：M070-1
品名：Emfret FITC-labeled Rat Anti-Mouse Integrin a2 Chain (CD49b)
規(guī)格：1.5 ml / 300 tests
同種型：Sam.G4 / Rat (Wistar) IgG2b
包含：FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5%
BSA and 0.09% (w/v) sodium azide
特異性：
The Sam.G4 antibody reacts with the mouse integrin a2 chain (CD49b), the 160-kDa
transmembrane glycoprotein that non-covalently associates with the integrin b1 subunit to form the
integrin a2b1 complex known as VLA-2. Integrin a2b1, a receptor for collagen and laminin, is
expressed on platelets, epithelial cells, and activated lymphocytes1,2. The Sam.G4 antibody can block
integrin a2b1 binding to collagen3
.
用途：
: The antibody preparation is optimized for flow cytometric applications: Use 5 μl to stain ~106
platelets or ~0.5x106 cells in a recommended volume of 25 μl. Incubate for 15 minutes at room
temperature, stop reaction by addition of 400 μl PBS and analyze samples within 30 minutes.
For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be
determined individually.
制備和儲存：
The antibody was purified from hybridoma cell culture supernatant by
Protein G-Sepharose chromatography. The antibody was conjugated with FITC under optimum
conditions. The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged
exposure to light. Stable for one year from date of shipment. Do not freeze.
注意：
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations
containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may
be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free
buffer.
注：僅供研究使用，不用于診斷或治療用途。
