Human Neutrophils Rolling on Human Endothelial Cells Expressing E-selectin
Parallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and/or reagents.
(Refer to Protocol 7)
Circular Flow Chamber (for 35mm tissue culture dishes)
31-001
Circular Flow Chamber Kit ($675.00)
Technical Reference: Improvements to Parallel Plate Flow Chambers to Reduce Reagents and Cellular Requirements, Brown and Larson, BMC Immunology 2001, 2:9
Flow Chamber Kit Includes:
Two flow decks (cast acrylic with different inlet/outlet designs)
8 silicon rubber gaskets (two of each size) Flow Width = 0.25 cm, Thickness = 0.005 in Flow Width = 0.25 cm, Thickness = 0.010 in Flow Width = 0.50 cm, Thickness = 0.010 in Flow Width = 1.00 cm, Thickness = 0.010 in
24 polypropylene tubing connectors(six of each) Threaded 1/16 in. straight and L-connectors Male and Female taper luer to 1/16 in. connectors
Silastic? laboratory tubing (1/16 in. ID x 10 ft.)
Rectangular Flow Chamber (for standard microscope slide)
31-010
Rectangular Flow Chamber Kit
$625.00
One flow deck (cast acrylic)
4 silicon rubber gaskets (two of each size) Flow Width = 1.00 cm, Thickness = 0.005 in Flow Width = 1.00 cm, Thickness = 0.010 in
Silastic? laboratory tubing(1/16 in. ID x 10 ft.)
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