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Parallel Plate Flow Chambers  

Human Neutrophils Rolling on  Human Endothelial Cells Expressing E-selectin

Parallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and/or reagents. (Refer to Protocol 7)

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Circular Flow Chamber (for 35mm tissue culture dishes)

31-001 Circular Flow Chamber Kit ($675.00)   Technical Reference: Improvements to Parallel Plate Flow Chambers to Reduce Reagents and Cellular Requirements, Brown and Larson, BMC Immunology 2001, 2:9

Flow Chamber Kit Includes: Two flow decks  (cast acrylic with different inlet/outlet designs) 8 silicon rubber gaskets (two of each size) Flow Width = 0.25 cm, Thickness = 0.005 in Flow Width = 0.25 cm, Thickness = 0.010 in Flow Width = 0.50 cm, Thickness = 0.010 in Flow Width = 1.00 cm, Thickness = 0.010 in 24 polypropylene tubing connectors(six of each)  Threaded 1/16 in. straight and L-connectors Male and Female taper luer to 1/16 in. connectors Silastic? laboratory tubing (1/16 in. ID x 10 ft.)

Replacement Gaskets and Coverslips for the Circular Flow Chamber
31-002	Four Gaskets  (Flow Width = 0.25 cm, Thickness = 0.005 in)	$30.00
31-003	Four Gaskets  (Flow Width = 0.25 cm, Thickness = 0.010 in)	$30.00
31-004	Four Gaskets  (Flow Width = 0.50 cm, Thickness = 0.010 in)	$30.00
31-005	Four Gaskets  (Flow Width = 1.00 cm, Thickness = 0.010 in)	$30.00
31-008	Circular Glass Coverslips (pack of 30, 35 mm, #2)	$30.00

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Rectangular Flow Chamber (for standard microscope slide)

31-010 Rectangular Flow Chamber Kit  $625.00

Flow Chamber Kit Includes: One flow deck (cast acrylic) 4 silicon rubber gaskets (two of each size) Flow Width = 1.00 cm, Thickness = 0.005 in Flow Width = 1.00 cm, Thickness = 0.010 in 24 polypropylene tubing connectors(six of each)  Threaded 1/16 in. straight and L-connectors Male and Female taper luer to 1/16 in. connectors Silastic? laboratory tubing(1/16 in. ID x 10 ft.)

Replacement Gaskets for the Rectangular Flow Chamber
31-011	Four Gaskets  (Flow Width = 1.0 cm, Thickness = 0.005 in)	$30.00
31-012	Four Gaskets  (Flow Width = 1.0 cm, Thickness = 0.010 in)	$30.00