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PURE - anti mouse CD62L - Clone MEL-14

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PURE - anti mouse CD62L - Clone MEL-14

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PURE - anti Mouse CD62L - MEL-14

Reference	Size	Price	Quantity	Add to cart
MO62LPU(V100)	100 μg	74,00 € tax excl.	The minimum purchase order quantity for the product is 1
MO62LPU(V500)	500 μg	172,00 € tax excl.	The minimum purchase order quantity for the product is 1

Reference

Size

Price

Quantity

Add to cart

MO62LPU(V100)

100 μg

74,00 € tax excl.

MO62LPU(V500)

500 μg

172,00 € tax excl.

Product Description

Clone: MEL-14
Isotype: Rat IgG2a
Tested Applications: Flow Cytometry
Conjugated: PURE
Concentration: 0.5 mg/ml
Immunogen: C3H/eb mouse B lymph oma 38C-13
Species Reactivity: Mouse
Storage Buffer: The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
Regulatory Status: RUO
Recommended Usage: CD62L, clone MEL-14, is a mAb intended for RUO in the identification of cells that express CD62L. This reagent is effective for direct IF staining of mouse sample for FCM analysis using ≤1 μg/106 cells.
Presentation: Liquid
Storage Instruction: Store in the dark at 2-8 °C

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Large description: CD62L (L-selectin), binds a 95 kDa (on neutrophils) or 74 kDa (on lymph ocytes) receptor with lectin-like and Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T lymph ocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for lymph ocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from lymph ocytes and neutrophils upon cellular activation; metalloproteinases may mediate the release of CD62L ectodomains from the cell surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, may be ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymph ocytes to peripheral lymph node HEV and inhibits in vivo lymph ocyte extravasation into peripheral lymph nodes and late stages of leukocyte rolling.

Other names: Sell; L-selectin; LECAM-1; LAM-1; Lnhr; Ly-22; Ly-m22; Lyam-1
GeneID: 20343
Molecular weight: 95 kD (neutrophils) or 74 kD (lymphocytes)