Antigen details
Reactivity:? The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones.
The CD8 monoclonal antibody is directed against the CD8-antigen (T8-antigen),which is expressed on human T lymphocytes. The monoclonal antibody reacts with 20-30% of human peripheral T lymphocytes. The monoclonal antibody reacts with T lymphocytes with suppressor-cell activity in pokeweed mitogen- stimulated immunoglobulin production, as was shown in separation experiments (i.e. "panning"). The monoclonal antiody does not react with B-cells, monocytes, granulocytes and platelets.
Specificity: The CD3 antibody recognizes 22/26/30 kD MW lymphocyte surface molecules associated with the T cell antigen receptor complex. Reacts with 85% of peripheral blood T lymphocytes, 70% of thymocytes, the majority of T cell chronic lymphocytic leukaemias, Sezary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.
The CD8 antibody recognizes 30/32 kD MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.
