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Activated Protein C PRODUCT INQUIRY Inactivation of Factor Va by Activated Protein C (APC) Proteolytic inactivation of factor Va by APC is represented, where: PS=protein S, PCPS=phospholipid vesicles (or cellular surface) and Ca++=calcium ions. The inactivation of factor Va to form factor Vai results from proteolysis of the factor Va heavy chain (94K) at three specific sites by APC (solid arrows) (1,2). The location of these cleavage sites in the factor Va heavy chain are as follows: (human: R306, R506, & R679) and (bovine: R306, R505, & R662). Complete inactivation of the cofactor molecule requires cleavage at the Arginine-306 position. Cleavage at Arginine-306 by activated protein C occurs only in the presence of membrane, and requires prior cleavage of the heavy chain at Arginine-505. Proteolysis of the factor Va light chain by APC occurs only in the bovine molecule and is not required for inactivation (dashed arrow). HCAPC-0080 Human Activated Protein C Pricing: Please Inquire SAMPLE DATA SHEET | SAFETY DATA SHEET Size 50 μg Formulation 50% (vol/vol) Glycerol/H2O Storage -20°C Purity >95% by SDS-PAGE Activity Determination Chromogenic Assay Shelf Life (properly stored) 12 months HCAPC-DEGR Human Activated Protein C - DEGR (active site blocked) Pricing: Please Inquire SAFETY DATA SHEET Size 50 μg Formulation 20 mM Tris, 150 mM NaCl, pH 7.4 Storage -80°C Purity >95% by SDS-PAGE Activity Determination Chromogenic Assay Shelf Life (properly stored) 12 months BCAPC-1080 Bovine Activated Protein C Pricing: Please Inquire SAFETY DATA SHEET Size 50 μg Formulation 50% (vol/vol) Glycerol/H2O Storage -20°C Purity >95% by SDS-PAGE Activity Determination Chromogenic Assay Shelf Life (properly stored) 12 months BCAPC-DEGR Bovine Activated Protein C - DEGR (active site blocked) Pricing: Please Inquire SAFETY DATA SHEET Size 50 μg Formulation 20 mM Hepes, 150 mM NaCl, pH 7.4 Storage -80°C Purity >95% by SDS-PAGE Activity Determination Chromogenic Assay Shelf Life (properly stored) 12 months