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Human CD45RA is expressed on all cells of hematopoietic origin, except erythrocytes. CD45RA is a transmembrane tyrosine phosphate which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RA isoform predominates on naive/resting T cells and medullary thymocytes.

Target	CD45RA
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Tissue / cell preparation (Human). Fresh human lymph node lymphocytes depleted of surface immunoglobulin positive cells were used as the immunogen. This antigen is a single chain glycoprotein which is expressed on B cells, monocytes, and T cell subsets.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG1
Conjugation	CF-Blue
Specificity	The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral Tlymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100mAb as a phenotypic marker to discriminate T cell subsets.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	220 kDa
Swiss Prot	P08575
GeneID	5788
Enzyme Commission Number	EC 3.1.3.4
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.