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CD20 is present on all normal B cells in peripheral blood, lymph nodes, spleen, tonsil and bone marrow. The antigen is also present on most B cell lines and on non-T ALL and CLL cells.

Target	CD20
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Normal human lymphocytes from lymph node.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG2a
Conjugation	CF-Blue
Specificity	CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	37/35 kDa
Swiss Prot	P11836
GeneID	931
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.