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Phospho-COT (Thr290) Antibody detects endogenous levels of COT only when phosphorylated at Threonine 290. This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways.

Target	COT (pT290)
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB, IHC, IF/ICC
Recommended dilutions	WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	A synthesized peptide derived from human COT around the phosphorylation site of Threonine 290.
Purification	Immunogen affinity purified.
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	60 kDa
Swiss Prot	P41279
Enzyme Commission Number	EC 2.7.11.25
Buffer	Rabbit IgG in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.