GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.? These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.? GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.? GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.? Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.? The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.? Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.
Source:
E.coli
Synonyms:
Glial-Derived Neurotrophic Factor, ATF-1
AA Sequence (monomer):
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Purity:
≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.
