FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant Murine FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
Source:
E.coli
Synonyms:
Fibroblast Growth Factor-acidic, FGF-1, HBGF-1, ECGF-beta
AA Sequence:
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Purity:
≥ 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ≤ 0.5 ng/ml corresponding to a specific activity of ≥ 2 x 106 units/mg.
Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.2 ng/ml in the presence of 10 μg/ml heparin, corresponding to a specific activity of ≥ 5 x 106 units/mg.
