B-? and? T-lymphocyte? attenuator? (BTLA),? or? CD272,? is? a? type? I? transmembrane? co-inhibitory? receptor? of? the? CD28? receptor? family? that? is? involved? in? modulating? adaptive? immune? responses? and? T? cell? co-stimulation.??? BTLA? acts? in? a? manner? similar? to? PD-1? and? CTLA-4,? the? other? inhibitory? receptors? of? the? CD28? family,? as? an? anti-inflammatory? molecule? crucial ?to? dampening? immune? response.? Expressed? most? prominently? in? T? cells,? including? Th1,? Th2,? CD4+,? and? CD8+? cells,? BTLA? can? also? be? found? in? B? cells,? NK? cells,? dendritic? cells? and? macrophages.? Ligation? of? BTLA? with? HVEM,? a ?member? of? the? TNF? receptor? super? family? (TNFRSF),? induces? tyrosine? phosphorylation? of? ITIM? and? ITSM? motifs? found? within? the? BTLA? cytoplasmic? domain,? resulting? in? association? with? SHP-1? and? SHP-2.? While? HVEM’s? interactions? with? the? ligands? LIGHT? and? Lymphotoxin-α? induce? a? powerful? stimulatory? immune? response,? the? interaction? of? BTLA? and? HVEM? attenuates? T? cell? proliferation? and? the? production? of? cytokines? including? IL-2,? IL-10? and? IFN-γ.? This? interaction? between? BTLA? and? HVEM? is? the? only? receptor-ligand? interaction? known? to? bridge? the? CD28? and? TNFR? families.??? Recent? studies? provide? evidence? that? engagement? of? BTLA’s? third? cytoplasmic? motif,? Grb2,? may? transmit? a? positive? signal,? suggesting? both? a? co-inhibitory? and? co-stimulatory? role? for? BTLA.? Dysfunction? of? the? BTLA? pathway? plays? a? role? in? the? pathogenesis? of? numerous? autoimmune? diseases? and? cancers.--The? naturally? occurring? human? BTLA? monomer? consists? of? a? 127-amino-acid? extracellular? domain,? a? 21-amino-acid? transmembrane? domain,? and? a? 111-amino-acid? cytoplasmic? domain.? PeproTech’s CHO cell-derived? Recombinant? Human? BTLA? Fc? is? a? glycosylated,? disulfide-linked? homodimer? of? 353-amino-acid-residues? whose? monomer? consists? of? a? 120-amino-acid? portion? of? the? extracellular? domain? fused? to? the? 231-amino-acid? length? Fc? portion? of? human? IgG? by? two? glycine? residues.? The? calculated? molecular? weight? of? monomeric CHO cell-derived? Recombinant? Human? BTLA? Fc? is? 39.8? kDa;? however,? due? to? glycosylation,? it? migrates? at? an? apparent? molecular? weight? of? approximately? 50-55? kDa? by? SDS-PAGE? analysis? under? reducing? conditions.
Source:
CHO cells
Synonyms:
B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, CD272
AA Sequence (monomer):
KESCDVQLYI KRQSEHSILA GDPFELECPV KYCANRPHVT WCKLNGTTCV KLEDRQTSWK EEKNISFFIL HFEPVLPNDN GSYRCSANFQ SNLIESHSTT LYVTDVKSAS ERPSKDEMAS GGPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK
Purity:
≥ 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Determined by its ability to bind recombinant Murine HVEM Fc in a functional ELISA.
