The E.coli derived recombinant multimer protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119. The protein is fused to a GST tag at N-terminus. (1mg/ml) 1.5M urea, 25mM Tris-HCl, pH 8.0, 0.2% Triton-X and 50% glycerol.HCV Core Genotype-1b antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.; HCV Core Genotype-1b protein was purified by proprietary chromatographic technique. Immunoreactive with sera of HCV-infected individuals.