The E.coli derived recombinant protein contains the HCV NS5 immunodominant regions, amino acids 2061-2302. The protein is fused with GST at N-terminus. 1.5M Urea, 25mM Tris-HCl pH 8, 50% glycerol and 0.2% Triton-X.HCV-NS5 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.; HCV-NS5 protein was purified by proprietary chromatographic technique. Immunoreactive with sera of HCV-infected individuals.