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Pfu-DNA Polymerase Recombinant

品牌：Peptides International

貨號(hào)：ENZ-265

規(guī)格：100U

貨期：

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Pfu-DNA Polymerase Recombinant

商品詳情參考文獻(xiàn) 相關(guān)資料

DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase.DNA Polymerase Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5′ to 3′ direction in the existence of magnesium. Pfu DNA Polymerase possesses 3′ to 5′ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended. 50mM Tris-HCl, pH 8.2, 1mM DTT, 0.1mM EDTA, 0.05% CHAPS and 50% glycerol. 1. Ideal for high-fidelity amplification. 2. 3'-5' exonuclease activity provides a low error rate. 3. One of the most thermostable DNA polymerases known. 4. Lack of extendase activity means no unwanted 3' overhangs. 5. Optimal for blunt-end PCR cloning. 6. Optimum temperature near 75°C. 7. 95% active after 1-hour incubation at 98°C.
Origin: E.coli.
Storage Conditions: -20 °C

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