For staining, use 10ml/1,000,000 cells. Titer for cytotoxicity should be determined by the investigator. Mouse IgG2b. Purified mouse LN cells (C57Bl/6 anti-BALB/c). Ion exchange column. This antibody is also available as unconjugated antibody and conjugated to FITC. 1mg/ml in PBS (after reconstitution). Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Recognizes most mouse H-2K haplotypes (k,p,q,r,s). Does NOT react with H-2kd. Mouse Antibody Monoclonal.