Apolipoprotein E3 (ApoE) is a 34kDa protein component of serum chylomicrons, VLDL, and HDL particles. ApoE mediates the binding, uptake, and catabolism of these particles as a result of interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is imperative in fatty acid homeostasis and memory formation. Polymorphisms encode 3 variants (ApoE2, 3, 4), which are differentially connected to the development of atherosclerosis and neurogenerative disorders, mainly Alzheimer's disease.
APOE3 Human Recombinant (19-317) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 306 amino acids and having a molecular mass of 35kDa.
The APOE is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminus and purified by proprietary chromatographic techniques.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2µm filtered solution in PBS.
Solubility: It is recommended to reconstitute the lyophilized APOE in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized APOE3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOE should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Biological Activity: Immobilized rHuApoE3 binds to rMuVLDLR with EC50 less than 0.4µg/ml.