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TP53I3 antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:3,000.vMouse IgG2a heavy chain and ? light chain. Anti-human TP53I3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TP53I3 amino acids 1-332 purified from E. coli. TP53I3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Mouse Anti Human Monoclonal.