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mEOS2 pAb

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mEOS2 pAb

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mEOS2 pAb

$325.00

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Product code: A010-mEOS2
Unit Size: 50 μg

Lyophilised rabbit polyclonal protein A affinity purified antibody

GFP was introduced as a tool for molecular biology over 20 years ago and is thought of as one of those most significant contributions to modern microscopy. Many related fluorescent proteins are subject to oligomerisation and photobleaching and as such, limit the resolution obtainable from their use. mEOS2 is a photoactivatable green to red photoconvertible fluorescent protein derived from Anthozoa. It has recently emerged and is set to make significant contributions to the enhancement of super resolution microscopy for the study of cellular ultra structure and protein trafficking. There are already a number of papers describing its use in the developing field of Photoactivated localisation microscopy (PALM) reversible saturable optical fluorescence transitions (RESOLFT), stochastic optical reconstruction microscopy (STORM) and three-dimensional high-resolution imaging (McKinney et al. 2009). A010-mEOS2 is a protein A affinity purified rabbit polyclonal antibody raised against purified mEOS2 expressed in E.coli and stains mEOS2 fusion proteins expressed in bacteria and mammalian cells in both western blotting and immunofluorescence microscopy applications.

Description	Lyophilised rabbit polyclonal protein A affinity purified antibody
Immunogen	Purified mEOS2 expressed as a GST fusion protein in BL21 Rosetta E.coli cells
Isotype	IgG
Purification	Protein A affinity purified
Species reactivity	N/A
Tested applications	WB
Recommended dilution	WB 1:10,000 for high expression, 1:1000 for low level expression
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 50 μl of 18 MΩ H2O for a 1mg/ml solution with stable buffering conditions. Aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised 4 °C Reconstituted -80 °C
Datasheet	A010-mEOS2
MSDS	A010-mEOS2
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.