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The new CAPTUREomeTM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.
Kit highlights include
References
Store at 4-8 0C.
K010-310-Protocol
K010-310-MSDS
There have been many recent advances in methodologies to detect the only reversible lipidation reaction of proteins, S-palmitoylation. This fatty acyl post translational modification has gained great interest in the research community as it is increasingly recognised as important in both health and disease. In this article I will briefly examine the development of the acyl exchange methodologies used by researchers to measure S-palmitoylation. I will focus in particular on the recently published Acyl PEG Exchange (APE) /Acyl PEGyl Gel Shift (APEGS) assay, paying particular attention to the advantages it offers scientists interested in exploring palmitoylation.
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