CaMKII (pThr286 or 287) pAb serum

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Product code: A010-50
Unit Size: 20 ul

A fully validated, highly specific rabbit polyclonal antibody to α and β isoforms of CaMKII Phospho Thr-286 (or Thr287 in β)

Ca²⁺/calmodulin-dependent kinase II (CaMKII) is a ubiquitous, multifunctional serine/threonine kinase involved in translating Ca²⁺- transient signals into cellular responses. Four separate CaMKII genes are expressed in man (α, β, δ, γ) with isoforms within each gene. CaMKII δ and γ are abundant in the heart. CaMKII is activated by Ca²⁺/CaM binding, and becomes activated for prolonged periods of time following autophosphorylation of Thr-286 or Thr-287 (equivalent residues in different isoforms). This antibody recognizes the active, autophosphorylated enzyme (all isoforms) and thus can measure the abundance of active CaMKII. Prolonged activation of CaMKII is associated with a variety of vascular and cardiac diseases including stroke, atrial fibrillation cardiac arrhythmia & sudden death, cardiac apoptosis, and heart failure.

Tested applications: western blot (1:5000 dilution) Species recognition: rat, human and mouse. Expected to react with various isoforms from chicken, cow, pig, rabbit, orangutan, zebrafish, ferret and Xenopus laevis.

Description	Lyophilised rabbit polyclonal anti-serum to CaMKII Phospho Thr-286/287.
Immunogen	Synthetic peptide (M281HRQET(PO3H2)VDC289) corresponding to amino acids surrounding the phosphorylated threonine residue at position 286 (or 287) of CaMKII, which was conjugated to keyhole limpet hemocyanin (KLH) by carbodiimide cross linkage.
Isotype	IgG
Purification	Raw serum
Species reactivity	Reacts with rat. Not yet tested in other species. However, from primary sequence analysis, the antibody will recognise Phospho Thr-286 (or 287) in all isoforms (α, β, γ and δ) from human, rat and mouse. The antibody is also expected to react with various isoforms from chicken, cow, pig, rabbit, zebrafish, and Xenopus laevis
Tested applications	WB
Recommended dilution	WB 1:5000
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 20 μl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised: 4°C Reconstituted:-80°C
Datasheet	A010-50
MSDS	A010-50
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.