Phospholamban (PLN, PLB) (pSer16) pAb

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Product code: A010-12AP
Unit Size: 50 μl

Affinity purified polyclonal antibody (IgG) to Serine-16 phosphorylated forms of phospholamban from the original polyclonal antiserum of Drago & Colyer (1994)

Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration. Phospholamban is phosphorylated on separate amino acid residues (Ser-16, Thr-17, Ser-10) by different kinases. cAMP-dependent, and cGMP-dependent protein kinases phosphorylate Ser-16. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure, underpin arrhythmias and provokes a change in phenotype of smooth muscle cells in atherosclerosis.

Tested applications: Western Blot (1:5000 dilution), IHC Microscopy (1:200 dilution). Not yet tested in other applications, therefore, optimal dilutions/concentrations should be determined by the user. Species recognition: All mammalian species, when phosphorylated on Ser-16.

Description	Lyophilised affinity purified Rabbit polyclonal antibody (A010-12AP) specific for Ser-16 phosphorylated forms of PLB (Drago & Colyer, 1994)
Immunogen	Phosphopeptide comprising residues 9-19-Y (residues R9SAIRRAS(PO3H2)TIE19Y) conjugated to KLH.
Isotype	IgG
Purification	Protein A affinity purified
Species reactivity	The antibody recognises mono and oligomeric phospholamban when phosphorylated on serine-16 by PKA. Binding of the antibody to its target epitope is blocked in the presence of a phosphopeptide containing the PLB Phospho Ser-16 epitope. Antibody affinity is reduced in circumstances of dual phosphorylation of Ser-16 and Thr-17.
Tested applications	WB , IHC
Recommended dilution	WB 1:5000, IHC 1:200
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 μl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised: 4 °C. Reconstituted:-80 °C
Datasheet	A010-12AP
MSDS	A010-12AP
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.