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iLite? Insulin Assay Ready Cells
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iLite? Insulin Assay Ready Cells is an avian DT-40 cell line (ATCC#CRL211) which has been genetically engineered, to be responsive to Insulin, resulting in the specific expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.
Incubation time
Drug Assays 30 min + 5 hours
NAb Assays 30 min + 30 min +5 hours
Detection system
Luminescence
Availability
Research use only
- Application
- Background
- Content
The iLite? Insulin Assay Ready Cells can be used to quantify insulin activity in human serum (without interference from factors present in human serum including IGF-1) and for quantification of the activity of neutralizing anti-Insulin antibodies.
Application Notes for the following assays are available:
? Quantification of Insulin using iLite? Insulin Assay Ready Cells (E-182-GB)
? Determination of neutralizing antibodies against Insulin using iLite? Insulin Assay Ready Cells (E-183-GB)
Insulin is a peptide hormone produced by beta cells in the pancreas to regulate the metabolism of carbohydrates and fats. Insulin is provided within the body in a constant proportion to remove excess glucose from the blood. When control of insulin levels fails, diabetes mellitus can result. As a consequence, insulin is used medically to treat some forms of diabetes mellitus.
Insulin exerts its activity by binding to a high affinity heterodimeric receptor, CD220, which possesses intrinsic tyrosine kinase activity. Binding of insulin to the insulin receptor alpha chain, results in receptor dimerization, receptor auto-phosphorylation, and signaling via the IR beta chain.
The insulin-like growth factor-1 (IGF-1) can also bind with low affinity to the IR and hence potentially interfere with an assay based on activation of the IR. Furthermore, the presence of insulin in serum samples could also interfere with the detection of neutralizing antibodies (NAbs). The iLite? Insulin Assay Ready Cells has been designed to eliminate interference from presence of insulin in serum samples, IGF-1 and IGF-2.
>250 μL of iLite Insulin Assay Ready Cells diluted in RPMI1640 with 20% heat inactivated bovine serum albumin and 10% DMSO.
