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BrightGreen 5X qPCR MasterMix-ROX

品牌：Abmgood

貨號：MasterMix-5R

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BrightGreen 5X qPCR MasterMix-ROX

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Description	Guaranteed high-performance real-time PCR using abm’s BrightGreen 5X qPCR MasterMix. abm’s BrightGreen 5X qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s BrightGreen 5X qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. Key Features ? Antibody hot-start technology provides superior specificity ? Enable streamlined protocol in a simple reaction set-up ? Allow accurate quantification of a variety of gene targets ? Reduce pipetting steps to minimize the risk of contamination ? Compatible with most real-time PCR instruments Please refer to our qPCR MasterMix Selection Guide (found in the Documents section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model.
Application	? Gene-expression analysis ? Gene knockdown validation ? Microarray validation ? High throughput applications ? Virus detection and quantification
Picture 1
Protocol
Storage	Stored at -20°C and protect from light.
Notes	1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles. 2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results. 3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range. 4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp. This product is ISO-13485 certified.