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BrightGreen miRNA qPCR MasterMix-No Dye

品牌：Abmgood

貨號(hào)：MasterMix-mS

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BrightGreen miRNA qPCR MasterMix-No Dye

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Description	BrightGreen miRNA qPCR MasterMix is designed specifically for quantitative real-time analysis of miRNA samples. The components of BrightGreen miRNA qPCR MasterMix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Form	MasterMix
Protocol
Concentration	2X
Storage	Upon arrival, BrightGreen miRNA qPCR MasterMix should be stored at -20oC and protected from light. After each experiment, the leftover thawed mix can be stored at 4oC if it is to be used within the next 3 months.
Notes	Primer concentration should not be high. 100nM to 300nM each - primer usually gives best results - Melting curve is useful in distinguishing nonspecific amplification products and primer dimers from desired product. It's recommended to run melting curve following amplification cycles. - To do replicates - To reduce pipetting error: preparing amplicon specific master mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range are very effective to get tight Ct. This product is ISO-13485 certified.