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Immortalized-Feline-Vena-Cava-Endothelial-Cells---SV40-&-hTERT

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Immortalized-Feline-Vena-Cava-Endothelial-Cells---SV40-&-hTERT

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Immortalized-Feline-Vena-Cava-Endothelial-Cells---SV40-&-hTERT

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	Feline
Source Organ	Vena cava inferior
Growth Properties	Adherent
Morphology	Endothelial-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Co-transduced with non-replicating lentivirus carrying SV40LT and hTERT
Description	Immortalized Feline Vena Cava Endothelial Cells - SV40 & hTERT are derived from primary endothelial cells isolated from the vena cava inferior of cats. The endothelium is involved in a variety of pathological processes, such as tumor invasion, atherosclerosis, arthritis, thrombosis, and vasculitis. With a feline model, scientists may use this model to further expand the knowledge in endothelial-related diseases in feline or can use this model to address to diseases in humans as well. The Immortalized Feline Vena Cava Endothelial Cells - SV40 & hTERT is a valuable source for long-term studies as the cells can be continuously cultured for experiments, and retains both the phenotype and characteristics as primary cells.
Procedure Overview
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 0.1 mg/ml gentamycin, 1 mM sodium pyruvate (TM057), 1% non-essential amino acids(TM068), 50 μg/ml endothelial cell growth supplement (ECGS; Biomedical Technologies Inc.), and 10 U/ml heparin (Sigma; H5515) and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	Immunodetection for hTERT and SV40L T antigen
Reference	Olyslaegers et al. (2013) Generation and characterization of feline arterial and venous endothelial cell lines for the study of the vascular endothelium. BMC Vet Res 9:170. DOI: 10.1186/1746-6148-9-170.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.