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Immortalized-Mouse-Spleen-Dendritic-Cells-(SRDC)-

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Immortalized-Mouse-Spleen-Dendritic-Cells-(SRDC)-

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Immortalized-Mouse-Spleen-Dendritic-Cells-(SRDC)-

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	Print Version
BioSafety Level	II
Organism	CBA/J Mouse
Source Organ	Spleen
Growth Properties	Adherent
Morphology	Dendritic-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	HuD, PGP9.5, tau, MHC-I, MHC-II, CD8, CD80, CD40, CD11c, CD59, CD205
Applications	For Research Use Only
Immortalization Method	Serial passaging and liposome-mediated transfection with pSV3neo plasmid carrying the SV40 T oncogene
Description	The Immortalized Mouse Spleen Dendritic Cell (SRDC) line is functionally and phenotypically similar to dendritic cells; specifically, in its antigen presentation, T cell priming, and dendritic cell marker expression. This innovative CD4 ^- CD8a ⁺ CD205 ⁺ CD11b ^- immortalized dendritic cell line eliminates the need for in vitro differentiation and expansion of different dendritic cell precursors into splenic dendritic cells. Therefore, these readily cultured cells are ideal for immunological studies focused on the function and biology of dendritic cells.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow V medium available from ABM (TM015). To make the completed growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999) to final concentration of 5%, 2mM L-glutamine, 50 μM β-Mercaptoethanol and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1. Immunofluorescence microscopy analysis confirmed SRDC maturation- through expression of DC-LAMP and MHC-II expression. 2. ELISA assays to determine interleukin and gamma interferon production. 3. To confirm dendritic cell transformation, SV40 large T antigen expression was compared between SRDCs and Cos-7-positive control cells and BHK-21-negative control cells. 4. PCR analysis confirmed that the SV40 oncogene was not integrated into SRDC cellular DNA. 5. Antigen uptake ability tested via incubation with antitoxoplasma serum.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.