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One-Step TaqProbe qRT-PCR-iCycler

品牌：Abmgood

貨號：G493-PC

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One-Step TaqProbe qRT-PCR-iCycler

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	Print Version
Description	Convenient real-time RNA quantification in one EASY step. abm’s One-Step TaqProbe qRT-PCR Kit uses a combination of high-quality enzymes in a proprietary buffer system to deliver precise and accurate sample analysis for high-throughput applications. This kit offers ultimate convenience in addition to consistent performance in terms of high sensitivity and superb signal-to noise ratio. While a one-step/single tube setup provides overall convenience and reduces room for error, abm’s One-Step TaqProbe qRT-PCR Kit offers additional advantages: ? Improved fidelity and yield for reverse transcription ? Prevention of template (RNA) degradation with RNaseOFF Ribonuclease Inhibitor. ? Superb performance with respect to sensitivity and signal-to-noise ratio. ? Significant reduction in non-specific PCR amplification by utilizing HotStart Taq DNA polymerase in the enzyme mix. Key Features ? Streamlined protocol in a simple, single-tube reaction set-up ? High-quality, full-length cDNA from as little as 0.01pg of RNA template ? Fully optimized for detection of low-copy genes ? Simple set-up for any RNA template (e.g. mRNA and total RNA) ? Reduces pipetting steps to minimize the risk of contamination and error. Please refer to our qPCR MasterMix Selection Guide (found in the Documents section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model.
Application	? Gene-expression analysis ? Transcription analysis ? Gene cloning ? High throughput applications ? Virus detection and quantification
Form	This kit includes 50X qRT-PCR Enzyme Mix and TaqProbe qPCR MasterMix
Picture 1
Protocol
Storage	Stored all components at -20°C. Protect the TaqProbe qPCR MasterMix from light.
Notes	1. Aliquot reagents to avoid risk of contamination and repeated freeze-thaw cycles. 2. Avoid high primer concentrations; a concentration of 100 nM to 300 nM of each primer usually gives the best results. 3. Probe concentration may require optimization, typically a 3:1 to 1:1 primer:probe ratio will give good amplification. 4. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: 1) preparing amplicon specific pre-mix, 2) using repeating pipettes and 3) keeping the pipetting volume within the manufacturer’s suggested range. 5. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp. This product is ISO-13485 certified.