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Immortalized-Human-Laryngeal-Posterior-Commissure-Cells

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Immortalized-Human-Laryngeal-Posterior-Commissure-Cells

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Immortalized-Human-Laryngeal-Posterior-Commissure-Cells

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Larynx
Growth Properties	Adherent
Morphology	Squamous
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	CK5, CK8
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene
Description	The posterior commissure region is made up of cells which aid in vocalization. More importantly, it is the region of the larynx most associated with the pharynx. This makes it the first area to be exposed to material from the stomach during laryngopharyngeal reflux (LPR); a mechanism suggested contributing to cancers and anomalies in the larynx. Due to its contact with LPR, posterior commissure cells are an attractive model to study laryngeal disorders. The Immortalized Laryngeal Posterior Commissure Cell Line retains its squamous phenotype and continues to express CK5 and CK8 markers after immortalization.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The medium for this cell line is Prigrow X.1 medium available in ABM, Cat. No. TM010. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. Temperature: 37.0°C.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.