Safe-Green? is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
Features
? Convenient: Safe-Green? is provided as a 6X loading dye, and is mixed directly with samples before gel loading. Inert tracking dye is included to monitor gel progress. ? Easy to Use: View and document your results as you would with EtBr staining. Safe-Green? can be excited with blue or UV light, and has maximum emission at 525 nm. ? Safe: Non-carcinogenic. ? Sensitive: Detect as little as 0.2 – 0.6 ng of DNA per gel band. ? Superior: EtBr is known to cause strand breaks and nicks in DNA. Using Safe-Green? minimizes such damage, yielding higher transformation rates and lower mutation rates verses EtBr. For even better cloning results use Safe-Green? with blue light excitation.
Application
Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Method Overview
Gel Image
Spectral Image
Storage
Store at 4°C for up to 2 years. Ships on blue ice.
Notes
Dispose Safe-Green? as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).
This product is ISO-13485 certified.