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Human-Normal-Pancreatic-Islets

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Human-Normal-Pancreatic-Islets

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	Print Version
Description	Human Primary Pancreatic islets are provided fresh upon order and shipped as live cells in a T25 flask. The Pancreatic islets consist a cluster of various hormone-producing cells, including 40%-60% pancreatic β cells. The percentage of the β cells varies between lot-to-lot preparations and can be determined as an added service. Upon cell arrival, it is recommended to use the cells within 7 days. Human primary Pancreatic islets is an ideal model for long-term islet grafting survival studies and can provide insight into development of novel therapies for other pancreas related diseases. Please contact our order desk for a quotation based on the IEQ required for your experiment. An IEQ or the “islet equivalent” is a standardized way to describe the islet size of 100 micron. In a case where a donor yields smaller islets, the customer may receive more actual islets than the ordered IEQ. The minimum IEQ per order is 1,000.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Pancreas
Growth Properties	Adherent
Morphology	Suspension
Applications	For Research Use Only
Disease	Normal
Propagation	The base medium for this cell line is Prigrow X series medium available in ABM. The media supplied during shipment is not sufficient for the cells and the customer will need to buy Cat. No. TM0199-A for IMMEDIATE Islet Recovery upon delivery and TM0199-B for culturing following recovery - Cat. No. TM0199-A for Islet Recovery as it enables the islets to round up with increased glucose responsiveness within the first 48 hours of recovery. - Cat. No. TM0199-B for Long Term Culturing as it is enhanced to reduce islet chaining and minimize islet fusion. - Cat. No. TM0199-C for Islet Cold Culture Media for transportation only. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Subculturing	Human Primary Pancreatic Islets can be split into different culture vessels (i.e. multi-well plates) for experimental needs, but they will not proliferate in vitro . Experiments should be conducted within 7 days upon cell arrival.
Preservation	Human Primary Pancreatic Islets cannot be cryopreserved.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.