丁香五月在线视频,99这里有精品视频视频,久久免费少妇高潮久久精品99

您好，歡迎來到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測(cè)溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁 > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Immortalized Human Alveolar Cells - Type 1

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized Human Alveolar Cells - Type 1

品牌：Abmgood

貨號(hào)：T0487

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購買加入購物車

Immortalized Human Alveolar Cells - Type 1

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Lung
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	5,000 cells per cm^2
Markers	CK-18, CK-19
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Cell Type Characterization
Description	Type I alveolar epithelial cells are large squamous cells, which extend across several air sacs and cover more than 95% of the alveolar surface area. They are specialized to serve as very thin (often only 25 nm in width) gas-permeable components of the blood-air barrier. The Type I Alveolar cells are the target of many insults, including ventilator-induced lung injury. These cells are useful in investigating the function and pathology of the respiratory system.
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in ABM, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). This cell line needs to be cultured in collagen coated flasks. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Quality Control	Real Time PCR to quantify transgene expression
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.