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EF1α Promoter mYamanaka Factors Lentivirus Set

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EF1α Promoter mYamanaka Factors Lentivirus Set

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EF1α Promoter mYamanaka Factors Lentivirus Set

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	Print Version
Vector	Refer to Individual Virus Pages
Plasmid Size	Refer to individual viruses.
Promoter	EF1α
Gene	mOct4, mSox2, mKLF4, mMyc and GFP control virus.
Organism	Mouse
Accession #	P20263, P48432, Q60793 and P01108
Description	Five Lenti-III-EF1α viruses, expressing the Yamanaka iPSC factor set (mOct4, mSox2, mKLF4 and mMyc) and a GFP control. Purified ready-to-use high titer lentivirus at 1x10⁷ cfu/mL
Functions	Each individual recombinant lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors provided in this set in combinantion with each other has been shown to reprogram ***** mouse fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).
Quality Control	Viral titer (IU/ml) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABM's viral preparations are tested to be free of bacteria and other microbials.
Titer	1x10⁷ IU/ml
Storage	-70°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar Induction of pluripotent stem cells from *** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and *** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.