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AZ-GR Stable HeLa Luciferase Reporter Cell Line

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AZ-GR Stable HeLa Luciferase Reporter Cell Line

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AZ-GR Stable HeLa Luciferase Reporter Cell Line

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	Cervix
Growth Properties	Adherent
Morphology	Epithelial-like
Description	The glucocorticoid receptpr (GR) is a ligand-activated transcription factor that binds endogenous glucocorticoids such as cortisol and synthetic glucocorticoids commonly used as anti-inflammatory or immunosuppressive drugs. The AZ-GR Stable HeLa Luciferase Reporter Cell Line is a selective, sensitive and reliable tool for high throughput gene reporter assays examining GR activity. It was created by stably transfecting plasmids containing glucocorticoid response elements (GREs) coupled with the luciferase reporter gene in to HeLa cells with endogenous GR. This cell line is particularly efficient for research on pre-clinical studies investigating novel glucocorticoid pharmaceuticals or for environmental applications, such as monitoring the levels of steroid hormones present in soil or water samples.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Donor Age	31
Donor Gender	Female
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in ABM, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 4 mM L-glutamine, 1% nonessential amino acids, 1mM sodium pyruvate and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
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Quality Control	Selective growth on media containing hygromycin B (0.2 mg/ml)
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.