色播情话深爱五月五月婷婷,五月激情综合免费观看,久999久久99久99久久精品视频

您好，歡迎來(lái)到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線(xiàn)客服QQ：3012681483 | 客服電話(huà)：400-968-7988 | 工作時(shí)間：08:30-17:30

主營(yíng)產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測(cè)溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁(yè) 產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁(yè) > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Mouse Primary Cochlear Perivascular Resident Macrophate-type Melanocytes (PVM/M

訂購(gòu)信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Mouse Primary Cochlear Perivascular Resident Macrophate-type Melanocytes (PVM/M

品牌：Abmgood

貨號(hào)：

規(guī)格：

貨期：

應(yīng)用：

咨詢(xún)客服

立即購(gòu)買(mǎi) 加入購(gòu)物車(chē)

Mouse Primary Cochlear Perivascular Resident Macrophate-type Melanocytes (PVM/Ms)

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Description	As part of the fluid-blood barrier in the stria vascularis, the perivascular resident macrophate-type melanocytes (PVM/Ms), along with cochlear microvascular endothelial cells and cochlear pericytes, are important for maintaining the inner ear homeostasis. The PVM/Ms have been found to be essential in regulating the permeability of the intrastrial fluid-blood barrier. With all three primary cells available, co-culture of the cell types is useful in studying the underlying causes of vascular dysfunction-related hearing loss.
Procedure Overview
BioSafety Level	II
Organism	C57BL/6J mice
Source Organ	Cochleae
Isolation	Mouse Primary Perivascular Resident Macrophate-type Melanocytes (PVM/Ms) were isolated and purified by flow cytometry based on F4/80 sorting. Sorted cells were validated with anti-F4/80 and anti-GSTα4 in immunohistochemical staining.
Growth Properties	Adherent
Morphology	Melanocyte-like, have unique dendritic processes
Image
Image
Markers	F4/80, CD68, and CD11b
Applications	For Research Use Only
Donor Age	10- to 15-day-old
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The basal medium for this cell line is 254CF Medium (Invitrogen, Cat No. M254CF500). To make the complete growth medium, add the following components to the basal medium: 10% fetal bovine serum (TM999), 1% human melanocyte growth factor (Life Technologies, Cat. No. S-002-5) and Penicillin/Streptomycin (G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%; Temperature: 37.0°C.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.