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Immortalized-Mouse-WildType-Aortic-Endothelial-Cells-(iMAEC-WT)

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Immortalized-Mouse-WildType-Aortic-Endothelial-Cells-(iMAEC-WT)

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Immortalized-Mouse-WildType-Aortic-Endothelial-Cells-(iMAEC-WT)

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	Print Version
BioSafety Level	II
Organism	Mouse
Source Organ	Heart
Growth Properties	Adherent
Morphology	Elongated
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	PECAM1, eNOS, VE-cadherin, and von Willebrand Factor
Applications	For Research Use Only
Immortalization Method	Immortalization using polyoma middle-sized T-antigen (PmT)
Description	The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) were derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of control mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. These cells provide solution in difficulty in maintaining mouse aortic endothelial cells in multiple passages without phenotypic drift.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) FACS cell sorting; 2) Characterization by Dil-Ac-LDL staining and immunostaining
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.