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Immortalized-Mouse-Epidermal-Cells-(COCA)

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Immortalized-Mouse-Epidermal-Cells-(COCA)

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Immortalized-Mouse-Epidermal-Cells-(COCA)

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	Adult Mice
Source Organ	Skin
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	0.5 – 1.5 x 10⁴ cells/ cm²
Markers	K5 (undifferentiated), K10 (early differentiation), loricrin and filaggrin (terminal differentiation)
Applications	For Research Use Only
Immortalization Method	Spontaneously immortalized in culture
Description	The Immortalized Mouse Epidermal Cells (COCA) retains its capacity to differentiate and stratify in response to increased calcium concentration and can form three-dimensional in vitro models. They form epidermis in grafting assays in vivo. It is resourceful as skin modeling and can be used for 3D epidermal models appropriate for skin biology research. Keratinocyte differentiation can be induced by switching the media to high calcium (1.2 mM) containing media.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X.1 medium available from abm (TM010). Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Quality Control	1) Functional assays such as the nitrate assay was performed to assess nitric oxide production; 2) ELISA was used to determine the TNFα secretion after inflammatory stimulation; 3) Western blot and immunocytochemistry were used to confirm the expression of microglial specific markers such as CD68 and Iba1 and M1/M2 phenotype-associated protein expression (COX-2/iNOS and Arg-1) after stimulation; 4) Aβ_1-42 or E. coli-derived bioparticles uptake was used to determine phagocytic activity of SIM-A9 cells.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.