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Immortalized-Mouse-Urogenital-Sinus-Mesenchymal-Cells-(UGSM-2)

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Immortalized-Mouse-Urogenital-Sinus-Mesenchymal-Cells-(UGSM-2)

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Immortalized-Mouse-Urogenital-Sinus-Mesenchymal-Cells-(UGSM-2)

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	Print Version
BioSafety Level	II
Organism	Mouse
Source Organ	Prostate
Growth Properties	Adherent
Morphology	Spindle-shape fibroblast-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	p16^INK4a and p19^ARF Knockout
Description	The Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2) are derived from a subline of the INK4a mouse, a transgenic knockout that lacks p16INK4a and p19ARF, which are specific inhibitors of cyclin-dependent kinases Cdk4 and Cdk6 that regulate cell cycle progression. UGSM-2 cells display a myofibroblast phenotype in culture with vimentin and smooth muscle actin expression, and is shown to respond to androgen stimulation. Co-culture of UGSM-2 cells with human prostate epithelium cells BPH-1, or co-grafting in vivo results in organized clusters of BPH-1 cells surrounded by a mantle of the UGSM-2 cells. The cells are responsive to Sonic hedgehog (SHH), an important signaling factor in prostate development, and mimic the transcriptional response of the intact UGS mesenchyme. This cell line is a useful model for studying the prostate stroma-epithelium signalling way, which plays an important role in prostate development and cancer progression.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, Gentamycin (50ug/ml) + 1% ITS + Glutamine (1x) + 10^-8M DHT, and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C
Quality Control	Real Time PCR was used to shows activation of SHH target genes
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.