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Print Version |
| BioSafety Level |
II |
| Organism |
Harlan Sprague-Dawley Rat Embryo |
| Source Organ |
Kidney |
| Growth Properties |
Adherent |
| Morphology |
Spindle-shaped |
| Recommended Seeding Density |
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Markers |
β-catenin, vWF, VEGFR-1, VEGFR-3, LYVE-1, vimentin, CD34, VCAM-1, ICAM-1, CD31 |
| Applications |
For Research Use Only |
| Immortalization Method |
Serial passaging and infection by Kaposi’s sarcoma-associated herpesvirus (KSHV) |
| Description |
Kaposi’s sarcoma-associated herpesvirus (KSHV) has been linked to several malignancies in AIDS patients, but the mechanisms involved are unknown. Past studies has postulated that KSHV targets precursor cells. Metanephric mesenchymal precursors are cells that are able to differentiate into different lineage of cells, including interstitial stroma, mesangial, vascular smooth muscle, cap mesenchyme, and loose mesenchyme cells.
The Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM) is suitable for KSHV infection related studies, such as the growth deregulation mechanisms, as it is able to cause tumor formation. The cells have been efficiently transformed and are immortalized by KSHV. It expresses vascular endothelial, lymphatic endothelial, mesenchymal, and hemapoietic precursor markers. There is downregulation of Thy1.1 expression, a tumor suppressor. These cells are recommended for research on cancer formation linked to KSHV.
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| Procedure Overview |
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| Propagation |
Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. |
| Quality Control |
Immunofluorescence assay, Western blot, and immunohistochemistry was preformed to detect cell markers |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
