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Immortalized-Rat-Schwann-Cells-(IFRS1)

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Immortalized-Rat-Schwann-Cells-(IFRS1)

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Fischer 344 Rat
Source Organ	Brain
Growth Properties	Adherent
Morphology	Spindle-shaped
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Spontaneous Immortalization
Description	Schwann cells are valuable tools for diabetic neuropathy pathogenesis involving glycation, reduced regenerative capability, oxidative stress, and other neurodegenerative diseases. The Immortalized Rat Schwann Cells express transcription factors (Krox20, Oct6, and SOX10), myelin proteins (P0, PMP22, and MAG), neurotrophic factors (NGF, GDNF, and CNTF), cell adhesion molecules (L1, NCAM, and N-Cadherin), and neurotrophin receptors (truncated TrkB, and TrkC), all of which points to distinct Schwann cell phenotype. Other glial markers such as S100, p75NTR, vimentin, GFAP, and laminin are also exhibited. These cells are unique in that they retain features of mature Schwann cells with the ability to myelinate neurites in co-culture with rat DRG neurons, thus providing a valuable tool to study neuron-Schwann cell interactions.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 20 ng/ml neuregulin-beta, 2 μM forskolin/DMSO, and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. Note: Growth of cells is dependent in cell density. The cells should be seeded in high density, otherwise their growth slows down.
Quality Control	Immunoreactivity to cell markers
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.