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Immortalized-Rat-Enteroglial-Cells-(EGC)

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Immortalized-Rat-Enteroglial-Cells-(EGC)

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Immortalized-Rat-Enteroglial-Cells-(EGC)

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	Print Version
BioSafety Level	II
Organism	Sprague-Dawley Rat
Source Organ	Jejunum
Donor Gender	Male
Growth Properties	Adherent
Morphology	Glial
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Serial passaging and infection by incubating with the supernatant of virus-producing GPE-egfr-neu cell line
Description	Enteroglial cells (EGCs) have a key role in the inflammatory response in the enteric nervous system which is structurally and functionally the largest and most diverse in complexity part of the peripheral nervous system. The Immortalized Rat Enteroglial Cells (EGC) is a valuable tool for studies in the enteric nervous system inflammatory response as it is an immortalized cell line established from the rat myenteric plexus. The cells express low levels of c-fos mRNA but can be up-regulated with stimuli from pro-inflammatory cytokines (IL-1β and TNF-α).
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. Propagation: Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 5% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. *To up-regulate c-fos* mRNA levels:** Stimulate cells for 60 minutes with either 10 ng/mL of IL-1β or 10 ng/mL of TNF-α. Split ratio: 1:4 or 1:6
Quality Control	Characterized by immunohistochemistry and dot-blot analysis.
Reference	A Rühl
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.